High Performance Liquid Chromatography
Separates and quantifies components in a liquid mixture using a pressurised mobile phase — the workhorse of pharma QC and R&D.
HPLC is a powerful analytical technique used to separate and quantify components in a liquid mixture based on their interaction with a stationary phase and a pressurised mobile phase.
It is widely used across the pharmaceutical industry for quality control and research & development applications.
How it works
Mobile phase flow
High-pressure pumps push solvent mixtures through the system, carrying the sample into the column.
Separation
Different interactions with the stationary phase result in varying elution rates, enabling separation.
Detection
Separated components are detected using UV, fluorescence and refractive index detectors.
Each component elutes at a different time due to its interaction with the stationary phase, allowing accurate identification and quantification.
At a glance
| Detectors | UV · Fluorescence · Refractive Index |
|---|---|
| Principle | Liquid–solid partition under high pressure |
| Service | Method development, validation & routine testing |
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